(-)-Epigallocatechingallate induces apoptosis in B lymphoma cells via caspase-dependent pathway and Bcl-2 family protein modulation

摘要: (−)-Epigallocatechingallate (EGCG) as a representative polyphenol has attracted increasing attention due to its diversified effects, especially potential an agent for the prevention or treatment of certain cancers. However, molecular mechanisms EGCG-induced apoptosis in B lymphoma cells are unclear. The aim this study was investigate effect EGCG on proliferation and cell lines Jeko-1 Raji, determine underlying mechanisms. Cell cytotoxicity were determined by counting kit (CCK-8) assay; assessed flow cytometry using Annexin V-PE/7AAD double staining; Fas, Bcl-2 Bax mRNA expression levels real-time PCR; caspase activity measured assay kit; apoptosis-associated proteins western blot analysis. We demonstrated that induced growth inhibition dose- time-dependent manner. In agreement, upregulated Fas while downregulating Bcl-2. Protein Bax, activated caspase-3, -7, -8, -9, PARP increased, protein reduced treatment. Taken together, induces triggering caspase-dependent intrinsic (mitochondrial) extrinsic (death receptor) pathways. These findings suggest may be lymphoma.