Polyamine Depletion Induces Rapid NF-κB Activation in IEC-6 Cells

摘要: The proliferation of the rat intestinal mucosal IEC-6 cell line requires polyamines, whose synthesis is catalyzed by enzyme ornithine decarboxylase (ODC). ODC inhibition leads to polyamine depletion, as well both and apoptosis regulating gene expression. NF-κB transcription factor regulates genes involved in apoptotic, immune, inflammatory responses. In present study we tested hypothesis that activated following inhibition. We found α-difluoromethylornithine (DFMO) resulted a ∼50% decrease intracellular putrescine levels within 1 h. DFMO through degradation inhibitory protein IκBα sequesters cytoplasm. DFMO-induced complexes contain p65 p50 members Rel family. activation was accompanied translocation from cytoplasm into nucleus. selectively inhibited reporter construct dependent on κB site HLA-B7 gene. contrast, had no effect interleukin-8 Thus, report activates factor, which may mediate altered physiological state cells occurs depletion.