circKIF4A promotes tumorogenesis of glioma by targeting miR-139-3p to activate Wnt5a signaling.

摘要: Glioma has the characteristics of high incidence and mortality, is a common malignant tumor central nervous system. Circular RNAs (circRNAs) have been reported to play vital roles in progression cancer including glioma, circKIF4A up-regulated glioma tissues. However, its role mechanisms gliomas are unclear. miR-139-3p were determined by qRT-PCR. Transwell assay, wound-healing cell colony formation flow cytometry performed measure invasion, migration, proliferation apoptosis. Western blotting was used evaluate Wnt/β-catenin pathway-related protein. Luciferase reporter assays confirmed relationship among circKIF4A, Wnt5a. Sphere ability glioma-initiating cells (GICs) spheroid formation. A nude mouse xenograft model established immunohistochemical staining detect Ki-67 Wnt5a levels. down-regulated both promoted expression sponging miR-139-3p. Knockdown inhibited ability, migration apoptosis regulating signaling pathway proliferation-related signal via Furthermore, knockdown or overexpression miR-139 suppressed sphere GICs inhibitd GICs. Additionally, depletion decreased level Ki-67, tumorigenesis modes. overexpressed miR-139-3p/Wnt5a axis. The results indicated that may be potential target for clinical treatment glioma.