Peripheral T cell expansion in lymphopenic mice results in a restricted T cell repertoire.

摘要: In the absence of thymic contribution, peripheral T cell pool is maintained by division mature lymphocytes. Recent studies suggest that expansion may be driven low-affinity interactions with self ligands. Here we have investigated consequence homeostatic proliferation on repertoire. Following day 3 thymectomy mice, there a subsequent 30-fold population. Significantly, population results in skewed TCR Vbeta complementarity-determining region (CDR)3 length distributions and, some cases, marked bias toward one or two CDR3 lengths. sequence analysis showed these biases were (oligo)clonal expansion. Neonatally thymectomized adult mice reduced antibody responses to primary challenge T-dependent antigens. These data demonstrate can result limited repertoire, indicating array stimulating ligands drives restricted.