Taxol production in suspension cultures of Taxus baccata
作者： Thomas J. Hirasuna , Luis J. Pestchanker , Venkatesh Srinivasan , Michael L. Shuler
DOI: 10.1007/BF00048185
关键词:
摘要: The response of Taxus baccata (PC2) to basic manipulations culture conditions is described. Suspension cultures were maintained at 25°C on a modified B5 medium with two-week transfers. Under these conditions, no taxol® formed. However, if the cells are left in same for 7 or more additional days, taxol produced and released (ca. 90%) into extracellular medium. Levels as high 13 mg 1−1 achieved shake flask was primary taxane formed representing between 50 80% total sensitive changes cycle through periods (13 1−1) low (<0.1 levels production during extended culture. Picloram most effective auxins tested respect cell growth but it suppressed production. Addition fructose moderately-productive 4 improved production, producing state did not respond. Glucose Two isoprenoids (geraniol pinene) had modest effect when added 10 1−1.
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