Rapid Isocratic Reversed Phase HPLC of Taxanes on New Columns Developed Specifically for Taxol Analysis
作者： Raymond E. B. Ketchum , Donna M. Gibson
DOI: 10.1080/10826079308019589
关键词: Elution 、 Reversed-phase chromatography 、 High-performance liquid chromatography 、 Isocratic hplc 、 Isocratic elution 、 Cephalomannine 、 Chemistry 、 Quantitative analysis (chemistry) 、 Resolution (mass spectrometry) 、 Chromatography
摘要: Abstract We describe a new HPLC method for detection of taxanes from crude extracts, using specialty columns developed analysis. Taxol [1] and cephalomannine [2] were separated in 13 minutes an isocratic elution. Additionally, there is baseline separation between [2], 7-epi-10-deacetyl-taxol [3], taxol [1]. Elution 7-epi-taxol [4] occurs 20 minutes. compared chromatography on specialty, C18, phenyl columns. This permits rapid analysis large numbers with excellent resolution taxanes, enabling the 7-epitaxol
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