Extraction, purification by solid-phase extraction and high-performance liquid chromatographic analysis of taxanes from ornamental Taxus needles

摘要: Needles from ornamental yews (Taxus spp.) are known to contain significant amounts of the anticancer agent Taxol (paclitaxel), as well other analogous taxane compounds. Methanol extracts taxanes needles efficiently, but crude methanolic extract contains large co-extractives in addition interest. The may be fractionally partitioned using C18 solid-phase extraction (SPE), permitting collection a fraction which elute quantitatively. This can then analyzed directly by means high-performance liquid chromatography. We have successfully scaled-up quantity SPE technique, larger cartridges prepared our laboratory Empore disks. disks permits us collect quantities for studies on its cytostatic effect non-mammalian eukaryotic cells.