Anti-proliferative effect of fungal taxol extracted from Cladosporium oxysporum against human pathogenic bacteria and human colon cancer cell line HCT 15
作者： K. Gokul Raj , R. Manikandan , C. Arulvasu , M. Pandi
DOI: 10.1016/J.SAA.2014.11.036
关键词:
摘要: Abstract Cladosporium oxysporum a new taxol producing endophytic fungus was identified and production of were characterized using UV–visible spectroscopy (UV–vis), high-performance liquid chromatography (HPLC), infrared (IR) nuclear magnetic resonance (NMR ( 13 C 1 H)) chromatography–mass spectrometry (LC–MS). The biosynthetic gene dbat ) evaluated for fungus. Antibacterial activity against six different human pathogenic bacteria done by agar well diffusion method. anticancer efficacy isolated fungal also in colon cancer cell HCT 15 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), cytotoxicity morphology analysis. showed positive towards effective both Gram as negative. suppress growth line with an IC 50 value 3.5 μM concentration 24 h treatment. Thus, the result reveals that C. could be potential alternative source have antibacterial properties possible clinical applications.
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