Separation of taxol from related taxanes in Taxus brevifolia extracts by isocratic elution reversed-phase microcolumn high-performance liquid chromatography
作者： S.D. Harvey , J.A. Campbell , R.G. Kelsey , N.C. Vance
DOI: 10.1016/0021-9673(91)85172-C
关键词:
摘要: Abstract Previous attempts to resolve the closely related taxanes cephalomannine and taxol by reversed-phase high-performance liquid chromatography (HPLC) on octadecyl silica columns have not been successful. In present study, high-resolution HPLC microcolumns packed with was evaluated for separation of aforementioned taxanes. Due enhanced efficiency this technique, baseline resolution between readily achieved under isocratic elution conditions. Chromatographic profiles Taxus brevifolia twig needle foliage extracts are presented that illustrate separation.
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Dennis C. Shelly, Jennifer C. Gluckman, Milos V. Novotny, Dead-volume free termination for packed columns in microcapillary liquid chromatography. Analytical Chemistry. ,vol. 56, pp. 2990- 2992 ,(1984) , 10.1021/AC00278A088
William P. McGuire, Taxol: A Unique Antineoplastic Agent with Significant Activity in Advanced Ovarian Epithelial Neoplasms Annals of Internal Medicine. ,vol. 111, pp. 273- 279 ,(1989) , 10.7326/0003-4819-111-4-273
Yukio Hirata, Column technology for packed capillary columns Journal of Microcolumn Separations. ,vol. 2, pp. 214- 221 ,(1990) , 10.1002/MCS.1220020503
Françoise Guéritte-Voegelein, Daniel Guénard, Pierre Potier, Taxol and derivatives: a biogenetic hypothesis. Journal of Natural Products. ,vol. 50, pp. 9- 18 ,(1987) , 10.1021/NP50049A002
Milos Novotny, Recent advances in the isolation and structural studies of biomacromolecules using microcolumn techniques Journal of Microcolumn Separations. ,vol. 2, pp. 7- 20 ,(1990) , 10.1002/MCS.1220020105
Toyohide Takeuchi, Daido Ishii, High-performance micro packed flexible columns in liquid chromatography Journal of Chromatography A. ,vol. 213, pp. 25- 32 ,(1981) , 10.1016/S0021-9673(00)80628-7
Richard D. Smith, Jose A. Olivares, Nhung T. Nguyen, Harold R. Udseth, Capillary zone electrophoresis-mass spectrometry using an electrospray ionization interface Analytical Chemistry. ,vol. 60, pp. 436- 441 ,(1988) , 10.1021/AC00156A013
Yukio Hirata, Milos Novotny, Techniques of capillary liquid chromatography Journal of Chromatography A. ,vol. 186, pp. 521- 528 ,(1979) , 10.1016/S0021-9673(00)95272-5
Franca. Andreolini, Claudio. Borra, Milos. Novotny, Preparation and evaluation of slurry-packed capillary columns for normal-phase liquid chromatography. Analytical Chemistry. ,vol. 59, pp. 2428- 2432 ,(1987) , 10.1021/AC00146A023
J. Fred Banks, Milos V. Novotny, A step gradient system for microcolumn liquid chromatography based on a multiple‐loop valve Journal of Microcolumn Separations. ,vol. 2, pp. 84- 87 ,(1990) , 10.1002/MCS.1220020208