Genetic diversity of Hungarian canine distemper virus strains.

摘要: To achieve proper diagnosis of dogs based on acute clinical symptoms and poorly preserved field samples taken from animals that died due to canine distemper (CD), a new differential diagnostic test has been developed polymerase chain reaction (PCR). In this study, more than 150 collected showing respiratory, gastrointestinal neurological signs suggesting virus (CDV) infection were examined. The consisted urine, blood nasal swabs clinically ill patients, sent our laboratory by clinicians various veterinary clinics throughout Hungary. Various organs during the necropsy with pathological changes suggested CDV also included. Three distinct PCRs designed. For purposes, primer pair specific 409 bases-long segment within conservative part large region (L) genome was Using test, out analyzed samples, 46 (30.66%) proved be positive for CDV, indicating still represents high risk population in phylogenetical analysis, completely encompasses hemagglutinin (H) gene amplicons sequenced both directions using appropriate primers. Our results indicate several different genotypes are currently present Nine Hungarian strains turned belong so-called Arctic group CDVs, most closely related non-European North America, China Greenland, as well phocine 2 (PDV-2) isolated Baikal seals (Phoca sibirica). One showed similarity other European isolates Denmark, Germany, Italy Turkey, geographically distant regions, such USA. seem join cluster is formed only couple strains, one mink another dog America. third set primers, restriction fragment length polymorphism (RFLP) assay designed fast reliable differentiation wild-type CDVs vaccine strains.