Long-Term Efficacy and Safety of Human Umbilical Cord Mesenchymal Stromal Cells in Rotenone-Induced Hemiparkinsonian Rats

摘要: Several studies have shown functional improvements, neuroprotective, and neuroregenerative effects after mesenchymal stem cells transplantation to parkinsonian animal models. However, questions remain about the safety, feasibility, long-term efficacy of this approach. In study, we investigated migration, therapeutic, tumorigenesis, epileptogenic human umbilical cord stromal (HUMSCs) 1 year into rotenone-induced hemiparkinsonian rats. Our data indicated that DiI-labeled HUMSCs migrated in lesioned hemisphere, from corpus striatum (CPu) substantia nigra. By integrating with host differentiating NSE, GFAP, Nestin, tyrosine hydroxylase-positive cells, prevented 48.4% dopamine neurons degeneration 56.9% terminals loss, both correlating improvement apomorphine-induced rotations. The CD50 CD97 value pentylenetetrazol semiquantitative immunohistochemical analysis proliferating cell nuclear antigen (PCNA), β-catenin, C-myc, NF-κB expression showed no significant difference between transplanted untransplanted groups, whereas expressions Bcl-2 P53 grafted CPu were upregulated by 281% 200% compared ungrafted CPu. results study suggest transplantation, most potential treatments for Parkinson's disease, is an effective safe