Intrinsic Signals for the Assembly of Hepatitis A Virus Particles

摘要: Capsid assembly is the final event of virus replication, and its understanding pivotal for design empty capsid-based recombinant vaccines drug delivery systems. Although capsid structure several members picornavirus family has been elucidated, little known about structural elements governing process that tightly associated with proteolytic processing viral polyprotein. Among picornaviruses, hepatitis A (HAV) unique in it contains VP1-2A as a component small protein VP4, which argues an pathway different from proposed other picornaviruses. Using system we show here HAV proteins' precursor P1-2A independent terminal domains 2A VP4 substrate. However, both play distinct roles particles. part primary signal pentameric structures only further aggregate to capsids when present N terminus precursor. Particle formation hepatovirus genus thus regulated by two intrinsic signals are those described