Single base mismatch detection by microsecond voltage pulses.

摘要: Abstract A single square voltage pulse applied to metal electrodes underneath a silicon dioxide film upon which DNA probes are immobilized allows the discrimination of targets with base mismatch during hybridization. Pulse duration, magnitude and slew rate all key factors controlling rates electric field assisted Although pulses 1 V, lasting less than 1 ms rise/fall times 4.5 ns led maximum hybridization fully complementary strands, lack stringency did not allow mismatches. However, by choosing conditions that slightly off optimum, selectivity for discriminating mismatches could be improved up factor ∼5 when was in middle strand ∼1.5 on 5′-end and. These results demonstrate appropriate provides new, site-specific, approach nucleotide polymorphisms sub-millisecond time scale, addressable microarrays.