Selective medium for culture of Mycoplasma hyopneumoniae.

摘要: The fastidious porcine respiratory pathogen Mycoplasma hyopneumoniae has proven difficult to culture since it was first isolated in 1965. A reliable solid medium been particularly challenging. Moreover, clinical and pathological samples often contain the fast-growing M. hyorhinis which contaminates overgrows primary culture. aim of this study optimise for recovery devise a selection from also containing hyorhinis. devised by Niels Friis improved use Purified agar incorporation DEAE-dextran. Addition glucose or neutralization acidity liquid with NaOH did not improve final yield viable organisms alter timing peak viability. Analysis relative susceptibility strains four antimicrobials showed that is less susceptible than kanamycin. This consistent all UK Danish tested. concentration 2μg/ml kanamycin selectively inhibited growth tested, while able grow. forms basis an effective selective hyopneumoniae.