Intravenous delivery of autologous mesenchymal stem cells limits infarct size and improves left ventricular function in the infarcted porcine heart.
作者： Ulf Krause , Christoph Harter , Anja Seckinger , David Wolf , Adrian Reinhard
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摘要: Systemic delivery of bone marrow–derived mesenchymal stem cells (MSCs) is a noninvasive approach for myocardial repair. We aimed to test this strategy in pig model infarction. Pigs (n = 8) received autologous MSCs (1 x 106/kg body weight) labeled with fluorescent dye 48 h post proximal left anterior descending artery (LAD) occlusion. Hemodyamics, infarct size, and function were assessed at baseline after 1 month. Morphologic analysis revealed that migrated the peri-infarct region, resulting smaller size (32 ± 7 vs. 19 7%, p 0.01), higher fractional area shortening (23 3 34.0 0.001), lower ventricular end diastolic pressure (18.7 5 10.2 4 mmHg, 0.02) +dp/dt (4,570 540 6,742 700 mmHg/s, 0.03) during inotropic stimulation. intravenous pigs limits an attractive tissue
liebertonline.com参考文章(36)
Sunil Rangappa, Vijay G. Reddy, Ariff Bongso, E. H. Lee, E. K. W. Sim, Transformation of the Adult Human Mesenchymal Stem Cells into Cardiomyocyte-Like Cells in Vivo Cardiovascular Engineering: An International Journal. ,vol. 2, pp. 7- 14 ,(2002) , 10.1023/A:1019974503258
A.A. Kocher, M.D. Schuster, M.J. Szabolcs, S. Takuma, D. Burkhoff, J. Wang, S. Homma, N.M. Edwards, S. Itescu, Neovascularization of ischemic myocardium by human bone-marrow–derived angioblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function Nature Medicine. ,vol. 7, pp. 430- 436 ,(2001) , 10.1038/86498
Donald Orlic, Jan Kajstura, Stefano Chimenti, Igor Jakoniuk, Stacie M. Anderson, Baosheng Li, James Pickel, Ronald McKay, Bernardo Nadal-Ginard, David M. Bodine, Annarosa Leri, Piero Anversa, Bone marrow cells regenerate infarcted myocardium Nature. ,vol. 410, pp. 701- 705 ,(2001) , 10.1038/35070587
Jizong Gao, James E. Dennis, Raymond F. Muzic, Magnus Lundberg, Arnold I. Caplan, The Dynamic in vivo Distribution of Bone Marrow-Derived Mesenchymal Stem Cells after Infusion Cells Tissues Organs. ,vol. 169, pp. 12- 20 ,(2001) , 10.1159/000047856
Wangde Dai, Sharon L. Hale, Bradley J. Martin, Jin-Qiang Kuang, Joan S. Dow, Loren E. Wold, Robert A. Kloner, Allogeneic Mesenchymal Stem Cell Transplantation in Postinfarcted Rat Myocardium Short- and Long-Term Effects Circulation. ,vol. 112, pp. 214- 223 ,(2005) , 10.1161/CIRCULATIONAHA.104.527937
M A Pfeffer, E Braunwald, Ventricular remodeling after myocardial infarction. Experimental observations and clinical implications. Circulation. ,vol. 81, pp. 1161- 1172 ,(1990) , 10.1161/01.CIR.81.4.1161
P Richard Vulliet, Melanie Greeley, S Mitchell Halloran, Kristin A MacDonald, Mark D Kittleson, Intra-coronary arterial injection of mesenchymal stromal cells and microinfarction in dogs The Lancet. ,vol. 363, pp. 783- 784 ,(2004) , 10.1016/S0140-6736(04)15695-X
Shinji Tomita, Donald A.G. Mickle, Richard D. Weisel, Zhi-Qiang Jia, Laura C. Tumiati, Yasmin Allidina, Peter Liu, Ren-Ke Li, Improved heart function with myogenesis and angiogenesis after autologous porcine bone marrow stromal cell transplantation. The Journal of Thoracic and Cardiovascular Surgery. ,vol. 123, pp. 1132- 1140 ,(2002) , 10.1067/MTC.2002.120716
Steven M. Devine, Amelia M. Bartholomew, Nadim Mahmud, Mary Nelson, Sheila Patil, Wayne Hardy, Cord Sturgeon, Terry Hewett, Theodore Chung, Wendy Stock, Dorie Sher, Scott Weissman, Karen Ferrer, Joseph Mosca, Robert Deans, Annemarie Moseley, Ronald Hoffman, Mesenchymal stem cells are capable of homing to the bone marrow of non-human primates following systemic infusion Experimental Hematology. ,vol. 29, pp. 244- 255 ,(2001) , 10.1016/S0301-472X(00)00635-4
Willy A Noort, Alwine B Kruisselbrink, Pieternella S in't Anker, Marjolein Kruger, Rutger L van Bezooijen, Roelf A de Paus, Mirjam H.M Heemskerk, Clemens W.G.M Löwik, J.H.Frederik Falkenburg, Roel Willemze, Willem E Fibbe, Mesenchymal stem cells promote engraftment of human umbilical cord blood–derived CD34+ cells in NOD/SCID mice Experimental Hematology. ,vol. 30, pp. 870- 878 ,(2002) , 10.1016/S0301-472X(02)00820-2