Relative rates of biosynthesis of collagen type I, type V and type VI in calf cornea

摘要: The biosynthesis of type I, V and VI collagens was studied by incubation calf corneas in vitro with [3H]proline as a marker. Pepsin-solubilized collagen types were isolated salt fractionation quantified SDS/PAGE. Expressed proportions the total hydroxyproline solubilized, corneal stroma comprised 75% 8% 17% collagen. rates incorporation, linear up to 24 h for each type, highest lowest I From pulse-chase experiments, calculated apparent half-lives 36 h, 10 6 respectively.