Corneal epithelialisation on surface-modified hydrogel implants: artificial cornea.

摘要: The objective was to investigate corneal re-epithelialisation of surface-modified polymethacrylate hydrogel implants in order evaluate them as potential materials for an artificial cornea. Polymethacrylate hydrogels were modified with amines and then coated different extracellular matrix proteins (collagen I, IV, laminin fibronectin). surgically implanted into bovine corneas maintained a 3-D culture system 5 days. epithelial growth across the implant surface evaluated using fluorescent, light electron microscopy. Full epithelialisation achieved on 1,4-diaminobutane-modified after coating collagen IV. Hydrogels 1,4-diaminobutane but without further only showed partial re-epithelialisation. other (1,2-diaminoethane or 1,3-diaminopropane) re-epithelialisation; improved these surfaces did not result complete Evaluation IV that remain clear, integrate well become covered by healthy stratified epithelium. In conclusion supported stable With refinement this has be used clinically