A comparison of biological coatings for the promotion of corneal epithelialization of synthetic surface in vivo.

摘要: PURPOSE To investigate the effect of a range biological coatings on corneal epithelialization synthetic polymer surface in vivo. METHODS Eight diverse factors (collagen I, collagen III, IV, laminin, fibronectin, endothelial extracellular matrix, hyaluronic acid, and chondroitin sulfate) were coated individually onto polycarbonate membranes with pore size 0.1 micro m. The implanted anterior cornea adult cats clinically assessed for rapidity extent persistence epithelial overgrowth. persistent attachment examined histologically by immunohistochemistry routine light electron microscopy. RESULTS Collagen laminin consistently enhanced migration cells Multiple-layered epithelium over I-, IV-, laminin-coated was demonstrated histologically. I-coated performed best, that they showed greater stratification differentiation epithelium. Formation basement membrane adhesion complexes detected microscopy up to 9 weeks after implantation. Membranes sulfate did not support Compromised biostability these mostly likely associated postsurgical reactions host tissue. CONCLUSIONS A biologically modified can I-modified exhibited most promising performance, both