Production of 1-decanol by metabolically engineered Yarrowia lipolytica.

摘要: Medium-chain alcohols are used to produce solvents, surfactants, lubricants, waxes, creams, and cosmetics. In this study, we engineered the oleaginous yeast Yarrowia lipolytica 1-decanol from glucose. Expression of a fatty acyl-CoA reductase Arabidopsis thaliana in strains Y. previously medium-chain acids resulted production 1-decanol. However, resulting titers were very low (<10mg/mL), most likely due product catabolism. addition, these produced small quantities 1-hexadecanol 1-octadecanol. Deleting major peroxisome assembly factor Pex10 was found significantly increase production, exceeding 500mg/L. It also increased 1-hexadecanoland 1-octadecanol titers, though increases less than those for These results demonstrate that can potentially be industrial other simple sugars.