Enhanced ethanol production through selective adsorption in bacterial fermentation

摘要: To alleviate the ethanol inhibition of Escherichia coli KO11 (ATCC 55124), during fermentation, online sequestration was achieved using F-600 activated carbon. Two separate schemes were tested, one involving direct addition carbon to fermentation flask for purpose in-situ adsorption and a second an externally located packed bed. For experiments, varying amounts adsorbent added medium at start fermentation. The in broth resulted increased glucose utilization production all flasks containing control flasks, approximately 75% available substrate utilized before inhibited. entire supply depleted. Ethanol also from 28 g/L no nearly 45 (including adsorbed phase) implementation bed adsorber on-line removal tested over number cycles evaluate performance productivity. Results indicate that maintaining concentrations below 20 ∼ 30 extends enhances After 3 period 180 h, additional 80% produced when compared despite suboptimal acidic pH medium.