Trypanosoma (Nannomonas) congolense : molecular characterization of a new genotype from Tsavo, Kenya

摘要: Trypanosoma (Nannomonas) congolense comprises morphologically identical but genetically heterogeneous parasites infective to livestock and other mammalian hosts; three different genotypes of this parasite have been described previously. Restriction enzyme fragment length polymorphisms (RFLPs) in both kinetoplast DNA minicircle nuclear sequences, randomly amplified polymorphic deoxyribonucleic acid (RAPD) patterns used here demonstrate the existence another type T. (N.) that is genotypically distinct from those so far characterized at molecular level. A highly repetitive, tandemly arranged sequence oligonucleotide primers, for use polymerase chain reaction (PCR) amplification are described, which can be specific identification trypanosome its distinction others within Nannomonas subgenus.