Expression of laminin by human fibroblasts, HT1080 fibrosarcoma cells and MCF-7 breast adenocarcinoma cells. Lack of regulation by the cell density and extracellular matrix.

摘要: We have cultured normal fibroblasts, fibrosarcoma HT1080 cells and breast adenocarcinoma MCF-7 on various substrates (plastic, collagen type I, laminin). All cell types used adhered the three with, however, a delayed attachment laminin. On all substrates, grew as monolayer with exception of that formed clusters The epithelial well mesenchymal (fibroblasts tumoral cells) synthesized laminin expressed mRNA coding for B1 chain 67 kD binding protein. levels these mRNAs were not modulated by culture conditions which affect morphology nor density.