Quantification and tracking of genetically engineered dendritic cells for studying immunotherapy.

摘要: Purpose Genetically encoded reporters can assist in visualizing biological processes live organisms and have been proposed for longitudinal noninvasive tracking of therapeutic cells deep tissue. Cells be labeled situ or ex vivo followed subjects over time. Nevertheless, a major challenge reporter systems is to identify the cell population that actually expresses an active reporter. Methods We used nucleoside analog, pyrrolo-2′-deoxycytidine, as imaging probe putative gene, Drosophila melanogaster 2′-deoxynucleoside kinase. Bioengineered were imaged animal models brain tumor immunotherapy using chemical exchange saturation transfer MRI. The number transduced was quantified by flow cytometry based on optical properties probe. Results We performed comparative analysis six different lines demonstrate utility mouse model immunotherapy. technology quantify given region, moreover sensitive enough detect less than 10,000 cells. Conclusion This unique enables efficient selection monitoring with both Magn Reson Med, 2017. © 2017 International Society Magnetic Resonance Medicine.