Codon-anticodon interaction studied with oligonucleotides containing 3-deazauridine, 4-deoxyuridine or 3-deaza-4-deoxyuridine: II. Ribosome binding of oligonucleotides and phenylalanyl-tRNA
作者： H.G. Gassen , H. Schetters , H. Matthaei
DOI: 10.1016/0005-2787(72)90511-4
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摘要: Abstract 1. Analogues of U-U-U in which every single uridine residue was replaced by either 3-deazauridine, 4-deoxyuridine, or 3-deaza-4-deoxyuridine were tested for their template activities stimulating the binding Phe-tRNA to ribosomes. All triplets containing inactive as templates, whereas replacements 3-deazauridine 4-deoxyuridine resulted a loss decrease activity depending on position substitution. 2. The 3 H-labelled oligonucleotides ribosomes dependent presence coded tRNA. 1 mole its analogue bound per tRNA Phe yeast . 3. Complex formations between and various measured equilibrium dialysis. Whereas (U) -U 4 showed constants 650 l · −1 3000 , respectively, complex formation other hardly detectable technique employed. 4. observations are discussed view mechanism aminoacyl-tRNA
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