RNA codons and protein synthesis

摘要: Substituting 5'-, 2'-, 3'-terminal or 2'-internal ribose hydroxyls of oligonucleotides markedly affected their template activity in directing the binding AA-sRNA to ribosomes. The relative oligo U preparations was as follows: p-5'-UpUpU > UpUpU CH3O-p-5'-UpUpU UpUpU-3'-p UpUpU-3'-p-OCH3 UpUpU-2',3'-cyclic phosphate. Trimers with (2'-5') phosphodiester linkages, (2'-5')-UpUpU and also (2'-5')-ApApA, did not serve templates for phenylalanine- lysine-sRNA, respectively. efficiency A p-5'-ApApA ApApA ApApA-3'-p ApApA-2'-p. hexamer, ApApApApApA considerably more active a than corresponding pentamer. These data indicate that two adjacent triplets are recognized by molecules bound nearby ribosomal sites. doublet 5'-terminal phosphate, pUpC, served serine-sRNA, whereas without terminal UpC, not. Although pUpC lower triplet UpCpU show serine-sRNA can recognize pUpC.