Mechanosensitivity of bone cells to oscillating fluid flow induced shear stress may be modulated by chemotransport.

摘要: Abstract Fluid flow has been shown to be a potent physical stimulus in the regulation of bone cell metabolism. In addition membrane shear stress, loading-induced fluid will enhance chemotransport due convection or mass transport thereby affecting biochemical environment surrounding cell. This study investigated role oscillating induced stress and cellular mechanotransduction mechanisms bone. Intracellular calcium mobilization prostaglandin E 2 (PGE ) production were studied with varying levels chemotransport. this MC3T3-E1 cells responded both an increase intracellular concentration ([Ca 2+ ] i PGE production. These responses modulated by The percentage responding [Ca oscillation increased increasing rate, as did . addition, depriving nutrients during resulted inhibition data suggest that yet determined factor media affects responsiveness even at constant peak stress. Chemotransport alone not elicit response, but it appears sufficient nutrient supply waste removal is needed for response