Delivery of retinoic acid to LNCap human prostate cancer cells using solid lipid nanoparticles
作者： Mushfiq H. Akanda , Rajeev Rai , Ian J. Slipper , Babur Z. Chowdhry , Dimitrios Lamprou
DOI: 10.1016/J.IJPHARM.2015.07.042
关键词:
摘要: In this study retinoic acid (RTA) loaded solid lipid nanoparticles (SLNs) were optimized by tuning the process parameters (pressure/temperature) and using different lipids to develop nanodispersions with enhanced anticancer activity. The RTA-SLN dispersions produced high-pressure homogenization characterized in terms of particle size, zeta potential, drug entrapment efficiency, stability, transmission electron microscopy (TEM), atomic force (AFM), X-ray diffraction (XRD) vitro release. Thermal analysis showed RTA be amorphous state, whilst microscopic images revealed a spherical shape uniform size distribution nanoparticles. Anticancer efficiency was evaluated incubating RTA-SLNs human prostate cancer (LNCap) cells, which demonstrated reduced cell viability increased concentrations (9.53% at 200 ug/ml) while blank SLNs displayed negligible cytotoxicity. cellular uptake SLN localization within cytoplasm cells flow cytometry indicated an increase fraction expressing early apoptotic markers, suggesting that are able induce apoptosis LNCap cells. have potential used for treatment.
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