Apoptosis-induced anticancer effect of transferrin-conjugated solid lipid nanoparticles of curcumin
作者： Rohit S Mulik , Jukka Mönkkönen , Risto O Juvonen , Kakasaheb R Mahadik , Anant R Paradkar
DOI: 10.1007/S12645-012-0031-2
关键词: Bioavailability 、 Zeta potential 、 Cytotoxicity 、 Pharmacology 、 Reactive oxygen species 、 Solid lipid nanoparticle 、 In vivo 、 Curcumin 、 Targeted drug delivery 、 Biophysics 、 Chemistry
摘要: Broad spectrum therapeutic potential of curcumin is usually hampered by its photodegradation and low bioavailability. Present investigation was designed with an objective to develop transferrin-mediated solid lipid nanoparticles (Tf-C-SLN) resistant the photostability capable enhancing bioavailability targeted drug delivery elicit anticancer activity against SH-SY5Y neuroblastoma cells in vitro. Hot homogenization method used for formulation Tf-C-SLN evaluated physicochemically using parameters such as, size, zeta potential, entrapment efficiency photostability, transmission electron microscopy (TEM), nuclear magnetic resonance (NMR), differential scanning colorimetry (DSC), vitro release study. In cytotoxicity apoptosis investigations were performed microplate analysis flow cytometry techniques. The physicochemical characterization confirmed suitability various therein. TEM revealed spherical morphology while NMR DSC study inside nanoparticles. cytotoxicity, reactive oxygen species, cell uptake found be increased considerably compared curcumin-solubilized surfactant solution, curcumin-loaded SLN (C-SLN) suggesting targeting effect. AnnexinV-FITC/PI double staining, DNA analysis, caspase detection, reduced mitochondrial induction nanoparticle treatment. Enhanced solution C-SLN observed from being major underlying mechanism. observations our are very compelling concrete advocate effect vivo possible clinical applications.
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