Matrix-assisted laser desorption of peptides and proteins on a quadrupole ion trap mass spectrometer
作者： Karen Jonscher , Graeme Currie , Ashley L. McCormack , John R. Yates
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摘要: The use of ultraviolet matrix-assisted laser desorption (MALD) to ionize peptides for analysis in a quadrupole ion trap is described. An source was modified accommodate fiber optic transmit radiation from notrogen (337 nm) the tip sample probe containing peptide protein samples matrix 2,5-dihydroxybenzoic acid (DHB) or 3,4-dimethoxy-4-hydrexy-cinnamic acid. Detection limits are demonstrated with 10 fmol sperm-whale-myoglobin. dimer sperm-whale myoglobin also observed at m/z 34, 430. A. comparison made tandem mass spectrum (MS/MS) human angiotensin I desorbed by MALD that liquid secondary-ion spectrometry. Both spectra were found contain abundant structural information.
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