Sildenafil citrate (Viagra) impairs fertilization and early embryo development in mice

摘要: Objective To determine the effects of sildenafil citrate, a cyclic monophosphate–specific type 5 phosphodiesterase inhibitor known to affect sperm function, on fertilization and early embryo cleavage. Design This acute mammal study included male female mice assigned randomly, females sacrificed after mating their oocytes/embryos evaluated at four time periods treatment. Setting Academic research environment. Animal(s) Male CBAB 6 mice. Intervention(s) Female were injected intraperitoneally with IU gonadotropin (hCG) stimulate follicular growth induce ovulation. They each caged that had been gavaged citrate (0.06 mg/0.05 mL) allowed mate. After 12, 36, 60, 84 h, killed, oviducts dissected out, retrieved embryos assessed for blastomere number quality. Main Outcome Measure(s) Fertilization rates numbers Result(s) (day 1) markedly reduced (−33%) in matings where taken citrate. Over days 2–4, developing treated group significantly fewer than control group. There was also trend impaired cleavage within those embryos, although this did not reach significance. Conclusion(s) The impairments fertility caused by have important implications infertility centers couples who are using drug precoitally while attempting conceive.