Topical application of TNF-α antiserum attenuates spinal cord trauma induced edema formation, microvascular permeability disturbances and cell injury in the rat
作者： Hari Shanker Sharma , T. Winkler , E. Stålberg , T. Gordh , P. Alm
DOI: 10.1007/978-3-7091-0651-8_85
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摘要: The possibility that antiserum to tumour necrosis factor-alpha (TNF-α) is neuroprotective in spinal cord injury (SCI) was examined a rat model. SCI produced by making an incision into the right dorsal horn at T10–11 segments. Topical application of TNF-a three concentrations (1:10; 1:50 and 1:100) given 30 min before or 2 min, 5 10 after trauma resulted marked reduction visible swelling, edema formation, leakage radiolabelled iodine tracer within T9 T12 segments h dose dependent manner. This effect most pronounced when highest level (1:10) applied SCI. also reduced induced upregulation neuronal nitric oxide synthase (nNOS) immunoreactivity concentration Taken together, these results suggest local TNF-α this mediated through NOS regulation.
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Hari Shanker Sharma, Jan Westman, Fred Nyberg, Pathophysiology of brain edema and cell changes following hyperthermic brain injury. Progress in Brain Research. ,vol. 115, pp. 351- 412 ,(1998) , 10.1016/S0079-6123(08)62043-9
Hari Shanker Sharma, Per Alm, Jan Westman, Nitric oxide and carbon monoxide in the brain pathology of heat stress. Progress in Brain Research. ,vol. 115, pp. 297- 333 ,(1998) , 10.1016/S0079-6123(08)62041-5
Weihong Pan, Abba J. Kastin, Richard L. Bell, Richard D. Olson, Upregulation of Tumor Necrosis Factor α Transport across the Blood–Brain Barrier after Acute Compressive Spinal Cord Injury The Journal of Neuroscience. ,vol. 19, pp. 3649- 3655 ,(1999) , 10.1523/JNEUROSCI.19-09-03649.1999
R.B. Borgens, J.J. Turek, I.A. Schoenlein, L.J. Moriarty, A. Leskovar, The macrophage in acute neural injury: changes in cell numbers over time and levels of cytokine production in mammalian central and peripheral nervous systems The Journal of Experimental Biology. ,vol. 203, pp. 1783- 1795 ,(2000)
H.S. Sharma, Y. Olsson, P.K. Dey, Early accumulation of serotonin in rat spinal cord subjected to traumatic injury. Relation to edema and blood flow changes Neuroscience. ,vol. 36, pp. 725- 730 ,(1990) , 10.1016/0306-4522(90)90014-U
Young B. Lee, Tae Y. Yune, Seoung Y. Baik, Young H. Shin, Shen Du, Hyewhon Rhim, Eunhee B. Lee, Young C. Kim, Moon L. Shin, George J. Markelonis, Tae H. Oh, Role of Tumor Necrosis Factor-α in Neuronal and Glial Apoptosis after Spinal Cord Injury Experimental Neurology. ,vol. 166, pp. 190- 195 ,(2000) , 10.1006/EXNR.2000.7494
P. ALM, B. LARSSON, EVA EKBLAD, F. SUNDLER, K.-E. ANDERSSON, Immunohistochemical localization of peripheral nitric oxide synthase-containing nerves using antibodies raised against synthesized C- and N-terminal fragments of a cloned enzyme from rat brain. Acta Physiologica Scandinavica. ,vol. 148, pp. 421- 429 ,(1993) , 10.1111/J.1748-1716.1993.TB09578.X
Yasuo Kuroda, Yoshinori Shimamoto, Human tumor necrosis factor-α augments experimental allergic encephalomyelitis in rats Journal of Neuroimmunology. ,vol. 34, pp. 159- 164 ,(1991) , 10.1016/0165-5728(91)90125-Q
H. S. Sharma, Y. Olsson, Edema formation and cellular alterations following spinal cord injury in the rat and their modification with p-chlorophenylalanine. Acta Neuropathologica. ,vol. 79, pp. 604- 610 ,(1990) , 10.1007/BF00294237
H.S. Sharma, Effect of captopril (a converting enzyme inhibitor) on blood-brain barrier permeability and cerebral blood flow in normotensive rats. Neuropharmacology. ,vol. 26, pp. 85- 92 ,(1987) , 10.1016/0028-3908(87)90049-9