Thyroid transcription factor-1 expression during normal human lung development and in patients with congenital diaphragmatic hernia

摘要: Background/Purpose: Thyroid transcription factor-1 (TTF-1) was detected in human respiratory epithelial cells from 11 weeks of gestation. TTF-1 is involved both lung morphogenesis and the regulation surfactant proteins. Recently, low expression nitrofen rat model congenital diaphragmatic hernia (CDH) shown restoration this downregulation by antenatal glucocorticolds (CS) reported. The aim study to investigate as a marker normal development age-matched controls specimen hypoplastic lungs CDH other forms hypoplasia. Methods: Immunohistochemistry monoclonal antibody performed on paraffin sections fetal neonatal tissues. so-called developmental group (12 weeks' gestation full term) included 47 specimens. hypoplasia 8 full-term patients who died within 12 hours after birth, 3 had CS therapy, 4 extracorporeal membrane oxygenation (ECMO) therapy. For comparison, 6 born patients, pulmonary hypoplasia, were used comparative specimen. Immunohistochemical localization evaluated light microscopy for different areas airways including intrapulmonary bronchi, intermediate terminal bronchioles, distal airways, later sacculi alveoli. Results: Nuclear staining observed progenitor developing bronchiolar early series. At term, expressed type II subsets nonciliated pattern similar all studied groups. No at level bronchi. Conclusions: difference neither lungs. This did not change with postnatal ECMO treatment. Although appears play an important role morphogenesis, pivotal likely. J Pediatr Surg 37:1258-1262. Copyright 2002, Elsevier Science (USA). All rights reserved.