Immunohistochemical characterization of canine neuroepithelial tumors.

摘要: The expression of cell differentiation and proliferation markers canine neuroepithelial tumors was examined immunohistochemically to identify the histogenesis these tumors. Astrocytomas (n = 4) consisted cells positive for glial fibrillary acidic protein (GFAP) nestin a few doublecortin (DCX). Immunoreactive receptor tyrosine kinases (epidermal growth factor c-erbB2) their downstream molecules (phospho-extracellular signal-regulated kinase 1/2 phospho-Akt) were often detected in astrocytomas, especially medium- high-grade Gliomatosis cerebri 3) ionized calcium-binding adaptor molecule 1 GFAP, including minor population nestin, DCX, beta III tubulin, suggesting differentiation. In choroid plexus 4), most tumor cytokeratins AE1/AE3 18, GFAP. majority oligodendrogliomas 5) DCX positive, but also contained populations or tubulin. Primitive neuroectodermal (PNETs; n 2) heterogeneous populations, neuronal major neuroblastoma tubulin single As antiapoptotic death molecules, tumors, PNETs, neuroblastomas intensely Bcl-2 Bcl-xL, whereas those gliomatosis almost negative. Bcl-xL-positive predominated over Bcl-2-positive cells, opposite observed oligodendrogliomas. immunohistochemical results analyzed by hierarchical clustering, constructed dendrogram clearly indicated novel position oligodendrogliomas: primitive