Functional up-regulation of KCNA gene family expression in murine mesenteric resistance artery smooth muscle.

摘要: This study focused on the hypothesis that KCNA genes (which encode KVα1 voltage-gated K+ channels) have enhanced functional expression in smooth muscle cells of a primary determinant peripheral resistance – small mesenteric artery. Real-time PCR methodology was developed to measure cell type-specific situ gene expression. Profiles were determined for arterial myocyte RNA species encoding subunits as well KVβ1, KVα2.1, KVγ9.3, BKCaα1 and BKCaβ1. The seven major expressed more readily detected endothelium-denuded artery compared with thoracic aorta; quantification revealed dramatic differential one two orders magnitude. There also four times KVα2.1 but less or similar amounts Patch-clamp recordings from freshly isolated dominant current density twice large cells. Therefore, we suggest increased production impacts physiological function, although there is quantitatively than might be expected. mechanism conferring up-regulated may common all family play role control blood pressure.