THE EFFECTS OF ENDOTOXIN ON VASCULAR ENDOTHELIUM

摘要: This study was undertaken to develop a technique for the preparation of sheets endothelial cells and investigate effects bacterial endotoxin on large numbers from continuous vascular endothelium. Rabbits were divided into one control two experimental groups. The animals received intracardially an LD(50) dose Escherichia coli endotoxin. 1 24 hr postinjection, vessels perfused with glutaraldehyde in Millonig's buffer methylene blue as marker. Pieces mesentery containing arteries postfixed buffered glutaraldehyde, dehydrated, placed acetone (to remove fat deposits). surrounding connective tissue stripped mesenteric arteries, segments slit longitudinally, flattened out, firmly affixed sheet cork fine mounting pins. A 3% solution Formvar ethylene dichloride pipetted onto luminal surfaces vessels. impregnated adhered and, after soaking overnight 10 N NaOH, could be vessel walls monolayers. Sheets Formvar-impregnated temporarily mounted glass slides aqueous examined by phase bright-field microscopy. Methylene stained nuclei deep cytoplasm faintly, but cell outlines indistinct. Endothelial rabbits had smooth, elliptical oriented parallel longitudinal axis irregularly distributed over smooth background faint striations. Essentially every endotoxin-injected appeared severely damaged. Cell posttreatment exhibited same type of, more extensive, damage than that observed animals. most prominent features damaged endothelium distorted nuclei, apparent nuclear vacuolization, missing nuclei. Unstained platelets plaques present specimens only. Stained unstained red blood also dispersed endotoxin-treated