Mutations of the p53 gene in the stool of patients with resectable colorectal cancer
作者： Susumu Eguchi , Norihiro Kohara , Koh Komuta , Takashi Kanematsu
DOI: 10.1002/(SICI)1097-0142(19960415)77:8<1707::AID-CNCR43>3.0.CO;2-0
关键词:
摘要: BACKGROUND. This study was undertaken to evaluate whether genetic analysis in the stool can be useful for detecting malignant tumors colon and rectum. We searched possible presence of mutations p53 gene patients with resectable colorectal cancer. Alterations are most frequent among mutant genes related METHODS. Surgically resected tumor specimens samples from 25 cancer were examined exons 5-8 by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP). Results compared those achieved fecal occult blood testing. RESULTS. Mutations found tissues 11 (44%). Of these patients, 7 (64%) had evidence alterations within stool. five who negative testing, evident three patients. CONCLUSIONS. method DNA tumor-specific is expected have a wide application clinical screening
sci-hub.se 参考文章(12)
J Bártková, B Vojtĕsek, J Lukás, J Bártek, D P Lane, C A Midgley, A Rejthar, Z Stasková, J Kovarík, J V Gannon, Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies. Oncogene. ,vol. 6, pp. 1699- 1703 ,(1991)
C. C. Boring, T. S. Squires, T. Tong, Cancer statistics, 1992 CA: A Cancer Journal for Clinicians. ,vol. 42, pp. 19- 38 ,(1992) , 10.3322/CANJCLIN.42.1.19
D Sidransky, T Tokino, Hamilton, K. Kinzler, B Levin, P Frost, B Vogelstein, Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors. Science. ,vol. 256, pp. 102- 105 ,(1992) , 10.1126/SCIENCE.1566048
Bernard Levin, Colorectal cancer screening. Cancer. ,vol. 72, pp. 1056- 1060 ,(1993) , 10.1002/1097-0142(19930801)72:3+<1056::AID-CNCR2820721319>3.0.CO;2-Z
M. Orita, H. Iwahana, H. Kanazawa, K. Hayashi, T. Sekiya, Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 2766- 2770 ,(1989) , 10.1073/PNAS.86.8.2766
M Hollstein, D Sidransky, B Vogelstein, C. Harris, p53 mutations in human cancers Science. ,vol. 253, pp. 49- 53 ,(1991) , 10.1126/SCIENCE.1905840
S. M. Caffarey, C. I. M. Broughton, C. G. Marks, Faecal occult blood screening for colorectal neoplasia in a targeted high‐risk population British Journal of Surgery. ,vol. 80, pp. 1399- 1400 ,(2005) , 10.1002/BJS.1800801114
Janice M. Nigro, Suzanne J. Baker, Antonette C. Preisinger, J. Milburn Jessup, Richard Hosteller, Karen Cleary, Sandra H. Signer, Nancy Davidson, Stephen Baylin, Peter Devilee, Thomas Glover, Francis S. Collins, Ainsley Weslon, Rama Modali, Curtis C. Harris, Bert Vogelstein, Mutations in the p53 gene occur in diverse human tumour types Nature. ,vol. 342, pp. 705- 708 ,(1989) , 10.1038/342705A0
D Sidransky, A VonEschenbach, YC Tsdi, P Jones, I Summerhayes, F Marshall, M Paul, PA Green, SR Hamilton, P Frost, B Vogelstein, None, Identification of p53 gene mutations in bladder cancers and urine samples Science. ,vol. 252, pp. 706- 709 ,(1991) , 10.1126/SCIENCE.2024123
David A. Ahlquist, Occult blood screening obstacles to effectiveness Cancer. ,vol. 70, pp. 1259- 1265 ,(1992) , 10.1002/1097-0142(19920901)70:3+<1259::AID-CNCR2820701511>3.0.CO;2-D