miR-3189-3p Mimics Enhance the Effects of S100A4 siRNA on the Inhibition of Proliferation and Migration of Gastric Cancer Cells by Targeting CFL2.

摘要: GDF15 is a downstream gene of S100A4. miR-3189 embedded in the intron GDF15—and coexpressed with it. miR-3189-3p functions to inhibit proliferation and migration glioblastoma cells. We speculated that S100A4 might regulate affect its function gastric cancer Quantitative reverse transcription polymerase chain reaction (qRT-PCR) showed expression was significantly downregulated MGC803 cells after knockdown. Overexpression inhibited Moreover, mimics enhanced effects an siRNA on inhibition cell migration. Dual luciferase reporter assays, qRT-PCR, Western blotting verified CFL2 direct target miR-3189-3p. mediates regulation Data mining based Kaplan–Meier plots high associated poor overall survival first progression cancer. These data suggested by targeting CFL2. The findings when treat cancer, consideration correction for counteracting factors should obtain satisfactory effect.