RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector
作者： DANQI CHEN , RUIXIU ZHANG , WEI SHEN , HAO FU , SHANSHAN LIU
关键词:
摘要: Our previous study using suppression subtractive hybridization (SSH), cDNA microarray and semi-quantitative RT-PCR showed that RPS12 was overexpressed in gastric cancer it closely related to metastasis. However, the role of is not clear, which led us conduct current further investigate effects on proliferation migration cells, also explore underlying molecular mechanisms. RNA interference used inhibit expression RPS12. The S100A4 cells determined western blot analysis. Cell were detected by MTT transwell assay, respectively. In addition, promoter activity measured a Dual-Luciferase Reporter Assay System. We found RNAi‑mediated downregulation reduced BGC823 SGC7901 cells. Further results inhibition decreased demonstrated ectopic reversed ability after findings provide first demonstration plays important roles regulating can mediate as downstream effector.
sci-hub.se 参考文章(29)
Lai Mao-De, Xu Jing, Ribosomal Proteins and Colorectal Cancer Current Genomics. ,vol. 8, pp. 43- 49 ,(2007) , 10.2174/138920207780076938
K Pogue-Geile, J R Geiser, M Shu, C Miller, I G Wool, A I Meisler, J M Pipas, Ribosomal protein genes are overexpressed in colorectal cancer : isolation of a cDNA clone encoding the human S3 ribosomal protein Molecular and Cellular Biology. ,vol. 11, pp. 3842- 3849 ,(1991) , 10.1128/MCB.11.8.3842
Hui Wang, Li-Na Zhao, Kai-Zong Li, Rui Ling, Xiao-Jun Li, Ling Wang, Overexpression of ribosomal protein L15 is associated with cell proliferation in gastric cancer BMC Cancer. ,vol. 6, pp. 91- 91 ,(2006) , 10.1186/1471-2407-6-91
C.-J Huang, C.-C. Chien, S.-H. Yang, C.-C. Chang, H.-L. Sun, Y.-C. Cheng, C.-C. Liu, S.-C. Lin, C.-M. Lin, Faecal ribosomal protein L19 is a genetic prognostic factor for survival in colorectal cancer. Journal of Cellular and Molecular Medicine. ,vol. 12, pp. 1936- 1943 ,(2008) , 10.1111/J.1582-4934.2008.00253.X
Yong-Wook Kwon, In Ho Chang, Kyung Do Kim, Young Sun Kim, Soon-Chul Myung, Mi-Kyung Kim, Tae-Hyoung Kim, Significance of S100A2 and S100A4 Expression in the Progression of Prostate Adenocarcinoma. Korean Journal of Urology. ,vol. 51, pp. 456- 462 ,(2010) , 10.4111/KJU.2010.51.7.456
D Chen, Z Zhang, M Li, W Wang, Y Li, E R Rayburn, D L Hill, H Wang, R Zhang, Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. Oncogene. ,vol. 26, pp. 5029- 5037 ,(2007) , 10.1038/SJ.ONC.1210327
Ruixiu Zhang, Hao Fu, Danqi Chen, Jun Hua, Yanping Hu, Kailai Sun, Xiuju Sun, Subcellular distribution of S100A4 and its transcriptional regulation under hypoxic conditions in gastric cancer cell line BGC823. Cancer Science. ,vol. 101, pp. 1141- 1146 ,(2010) , 10.1111/J.1349-7006.2010.01533.X
Sharon P. Shriver, Mark D. Shriver, Dayna L. Tirpak, Lillian M. Bloch, Jay D. Hunt, Robert E. Ferrell, Jill M. Siegfried, Trinucleotide repeat length variation in the human ribosomal protein L14 gene (RPL14): localization to 3p21.3 and loss of heterozygosity in lung and oral cancers. Mutation Research/Mutation Research Genomics. ,vol. 406, pp. 9- 23 ,(1998) , 10.1016/S1383-5726(98)00006-5
Birgitte Forst, Matilde Thye Hansen, Jörg Klingelhöfer, Henrik Devitt Møller, Gitte Helle Nielsen, Birgitte Grum-Schwensen, Noona Ambartsumian, Eugene Lukanidin, Mariam Grigorian, None, Metastasis-Inducing S100A4 and RANTES Cooperate in Promoting Tumor Progression in Mice PLoS ONE. ,vol. 5, pp. e10374- ,(2010) , 10.1371/JOURNAL.PONE.0010374
Jun Hua, Danqi Chen, Hao Fu, Ruixiu Zhang, Wei Shen, Shanshan Liu, Kailai Sun, Xiuju Sun, Short hairpin RNA–mediated inhibition of S100A4 promotes apoptosis and suppresses proliferation of BGC823 gastric cancer cells in vitro and in vivo Cancer Letters. ,vol. 292, pp. 41- 47 ,(2010) , 10.1016/J.CANLET.2009.11.007