Response surface models for effects of temperature, pH, and previous growth pH on growth kinetics of Salmonella Typhimurium in brain heart infusion broth.

摘要: Response surface models were developed for effects of temperature (15 to 40 degrees C), pH (5.2 7.4), and previous growth (5.7 8.6) on lag time (lambda) specific rate (mu) Salmonella Typhimurium in brain heart infusion broth (BHIB). Seventy-five curves model development 30 validation fit a two-phase linear obtain direct estimates lambda mu BHIB. natural logarithm transformations as function temperature, pH, obtained by regression analysis. Previous did not alter (P > 0.05) or interact with subsequent kinetics Typhimurium. However, BHIB affected < quadratic pH. The validated against data used their development. Mean absolute relative error predictions (model accuracy) was 7.8% 6.6% mu. Median bias) -1.8% -2.8% Results the current study indicated that accurately predicted within matrix factors modeled range investigated