Unravelling the Diversity of the Cyclopiazonic Acid Family of Mycotoxins in Aspergillus flavus by UHPLC Triple-TOF HRMS.

摘要: Cyclopiazonic acid (α-cyclopiazonic acid, α-CPA) is an indole-hydrindane-tetramic neurotoxin produced by various fungal species, including the notorious food and feed contaminant Aspergillus flavus. Despite its discovery in A. flavus cultures approximately 40 years ago, contribution to mycotoxin burden consistently minimized our focus on more potent carcinogenic aflatoxins also this fungus. Here, we report screening identification of several CPA-type alkaloids not previously found cultures. Our identifications these are based a dereplication strategy involving accurate mass high resolution spectrometry data careful study α-CPA fragmentation pattern. In total, 22 were identified extracts from strains examined. Of metabolites, 13 have been reported other fungi, though first their existence Two metabolite discoveries, 11,12-dehydro 3-hydroxy-2-oxo CPA, never for any organism. The conspicuous presence CPA numerous derivatives raises concerns about long-term cumulative toxicological effects secondary metabolites contributions entire problem.