The comparative efficacy of CTLA-4 and L-selectin targeted DNA vaccines in mice and sheep

摘要: The access of antigens to antigen presenting cells (APCs) appears be a rate-limiting step in the generation immune responses DNA vaccines. cytotoxic T lymphocyte 4 (CTLA-4) and L-selectin represent attractive ligands for use targeting APCs lymph nodes. CTLA-4 binds with high affinity B7 membrane on APCs, while functions as homing marker CD34 surface endothelial venule cells. vaccines encoding human immunoglobulin (HIg), fused either or L-selectin, have been shown generate up 10,000-fold higher anti-HIg antibody than HIg alone. In this study, ability mediated enhance humoral response an alternate vaccine was investigated. CTLA-4-HIg L-selectin-HIg host-protective 45W from Taenia ovis were constructed. BALB/c mice, targeted did not improve magnitude speed vaccinated mice. contrast, generated 45W-specific which 30-fold those achieved non-targeted vaccination. kinetic following vaccination also significantly faster that vaccination, adjuvanted protein Vaccination outbred sheep expressing murine ovine failed responses. These findings indicate may find application improvement vaccines, but requires further development applications large animal species.