The Combination of Catechin and Epicatechin Gallate from Fructus Crataegi Potentiates β-Lactam Antibiotics Against Methicillin-Resistant Staphylococcus aureus (MRSA) in Vitro and in Vivo

摘要: Fructus crataegi (hawthorn) is the common name of all plant species in genus Crataegus Rosaceae family. In present study, three monomers (+)-catechin (C), (−)-epicatechin gallate (ECg) and (−)-epigallocatechin (EGC) were isolated from hawthorn under guide antibacterial sensitization activity. The bioactivity composite fraction enhancing effect oxacillin against methicillin-resistant Staphylococcus aureus (MRSA) was greater than that individual monomer extract vitro. Two-fold dilution checkerboard methods used to analyze activity screen for combination proportion with best bioactivity. result showed C (128 mg/L) combined ECg (16 had greatest also reduced bacterial load blood septic mice challenged a sublethal dose MRSA, increased daunomycin accumulation within MRSA down-regulated mRNA expression norA, norC abcA, important efflux pumps MRSA. summary, enhanced β-lactam antibiotics vitro vivo, which might be related via suppression pumps’ gene expression.