EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE IN ENTEROBACTERIACEAE VI. High-Frequency Resistance Transfer System in Escherichia coli

摘要: Abstract Watanabe, Tsutomu (Keio University, Tokyo, Japan). Episome-mediated transfer of drug resistance in Enterobacteriaceae. VI. High-frequency system Escherichia coli. J. Bacteriol. 85:788–794. 1963.—R-factors (transmissible drug-resistance factors composed the resistance-transfer factor and markers to sulfonamide, streptomycin, chloramphenicol, tetracycline) gave rise a high-frequency coli K-12 F−, F+, Hfr strains that was similar colicinogeny (HFCT) found by Stocker Ozeki Salmonella typhimurium with colicinogenic I. The procedure for obtaining R-factors identical HFCT. majority cells were which had just received conjugation. These could act as highly competent donor further frequencies high 1.5 7.5 per resistant cell introduced into mixed incubation drug-sensitive recipient 1 hr at 37 C, whereas usual of, most, 1:30 under conditions cultivation. It also aerobic growth rather reduced systems. Possible mechanisms are discussed.