Comparison of autologous versus allogeneic epithelial-like stem cell treatment in an in vivo equine skin wound model

摘要: Abstract Background aims Several studies report beneficial effects of autologous and allogeneic stem cells on wound healing. However, no comparison between versus epithelial-like (EpSCs) has been made so far. For this reason, we first hypothesize that both EpSC types enhance healing in to vehicle treatment untreated controls. Second, the basis other studies, hypothesized there would be difference EpSCs. Methods Twelve full-thickness skin wounds were created six horses. Each horse was subjected (i) EpSCs, (ii) (iii) or (iv) control. Wound evaluation performed at day 3, 7 14 through exudates week 1, 2 5 biopsies. Results circumference surface significantly smaller EpSC-treated wounds. A lower amount total granulation tissue (overall) higher vascularization (week 1) observed after treatments. Significantly more major histocompatibility complex II–positive CD20-positive noticed 2. In groups, number EpSCs center biopsies low 1 (11.7% 6.1%), decreased 7.6% 1.7%, respectively 2), became undetectable week 5. Conclusions These results confirm hypothesis partially support second hypothesis. Besides macroscopic improvements, had similar formation, early cellular immune response.