[46] Chromatography on lipophilic sephadex

摘要: Publisher Summary This chapter describes methods for preparation of different lipophilic Sephadex derivatives that permit the creation straight- or reversed-phase partition and adsorption systems based on specific group interactions. Examples use some in preparative analytical chromatography are given. Column with is a valuable tool separation compounds soluble organic solvents. The technique has several advantages: mild conditions, high recoveries trace amounts labile compounds, simplicity use, possibility to prepare permanent column over long periods time. discusses synthetic procedures (1) nonselective derivatives—that is, materials polarity capable swelling solvents; (2) selective containing functional groups All reactions should be carried out moderate stirring avoid mechanical damage beads formation fines. A magnetic stirrer not suitable, it sufficient shake reaction vessel intermittently. If material narrow defined particle size required, starting sieved dry state.