Interrelationships between motility, c-AMP, respiration and calcium uptake of ram and boar sperm

摘要: Abstract Uptake of radioactive calcium by washed ejaculated ram and epididymal boar spermatozoa was inhibited theophylline dibutyryl cyclic AMP (diBc-AMP) which increases (c-AMP) levels in cells, the uncouplers 2,4-dinitrophenol (2,4-DNP) carbonylcyanide m-chlorophenylhydrazone (CCP), sodium azide, rotenone antimycin A ruthenium red La 3+ . Nicotine, eserine ouabain stimulated uptake caput sperm decamethonium it. Inhibition accompanied a decrease oxygen case metabolic inhibitors (rotenone, azide) substances that interfere with cell transport (ruthenium ). Respiration increased presence 2,4-DNP CCP, diBc-AMP. The phosphodiesterase (caffeine theophylline), diBc-AMP, 2,4-DNP, bicarbonate ion motility sperm. Most other depressed motility. ionophore A23187 severely an accompanying increase their largely unaffected addition inhibitors, activators surfactants. This may be explained operation competing mitochondrial plasma membrane pumps. However, selective permeability on polyene antibiotic filipin released accumulated A23187. provides further evidence for lack involvement influx produced ionophore.