Determination of polyacetylenes and ginsenosides in Panax species using high performance liquid chromatography.

作者: Daisuke Washida , Susumu Kitanaka

DOI: 10.1248/CPB.51.1314

关键词:

摘要: A new HPLC method was developed to separate and identify three polyacetylenes (panaxynol, panaxydol 1,8-heptadecadiene-4,6-diyne-3,10-diol) found in Panax species. The mobile phase a linear gradient of 2 : 1 3 (v/v/v) methanol/acetonitrile/water 40 min. analysis performed at flow rate 1.5 ml/min with UV detection 254 nm. contents the ginsenosides ginseng (white red ginseng), P. quinquefolium, japonicus, noteginseng were determined using these methods. species containing highest polyacetylene content (0.080%) quinquefolium cultivated Nagano, Japan. Meanwhile, ginsenoside (9.176%) Yunnan, China.

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