Characterization of human antibody to polyribophosphate, the capsular antigen of hemophilus influenzae, type B

摘要: Abstract The immunoglobulins involved in the human serologic response to polyribophosphate (PRP) 4 , capsular antigen of Hemophilus influenzae type b, have been studied. After immunization with 50 μg PRP, serum demonstrated primary antigen-binding capacity and a classic precipitin curve. Per unit weight, IgM from this was 100-fold more active than IgG PRP-specific hemagglutination 2-fold greater complement-dependent bactericidal activity. Specific anti-PRP antibody separated by immunoabsorption both assays; subgroups, only IgG1 detected preparation. high salt extract PRP precipitate hyperimmune serum, however, contained IgG2 ratio 1.6:1. Phagocytosis H. b vitro enhanced heated IgG, preparation but not IgM; activity approximately doubled addition complement. Inhibitor studies indicated that C 3 peptidase system.