Novel Chemosensitive Single-Nucleotide Polymorphism Markers to Targeted Regimens in Metastatic Colorectal Cancer
作者： Jin C. Kim , Seon Y. Kim , Dong H. Cho , Ye J. Ha , Eun Y. Choi
DOI: 10.1158/1078-0432.CCR-10-1907
关键词:
摘要: Purpose: Methods for predicting individual responsiveness to targeted chemotherapy are urgently needed, considering the frequent resistance and extremely high cost. Experimental Design: A chemosensitive single-nucleotide polymorphism (SNP) discovery schema is presented that utilizes (i) genome-wide SNP screening with a human array an in vitro chemosensitivity assay 118 colorectal cancers, (ii) clinical association analysis other 98 patients who had received metastatic cancer, (iii) biological utility assessment using cell viability assays of transfected cancer (CRC) cells. Results: Nine SNPs related bevacizumab cetuximab regimen sensitivity were chosen during screening. Overall responses regimens revealed carrying TT genotype at ANXA11 rs1049550 or least one G allele LINS1 rs11247226 seemed greater than those C AA genotype, respectively ( P GG DFNB31 rs2274159 LIFR rs3729740 = 0.025 0.07). Cytotoxicity analyses showed all RKO HCT116 CRC clones ISX rs361863 more sensitive T allele, ≤ 0.001–0.024). Conclusions: Chemosensitive markers identified novel three-step process. The candidate marker possibly will hopefully predict responsive regimens, although further validation needed large cohorts. Clin Cancer Res; 17(5); 1200–9. ©2011 AACR .
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kribb.re.kr参考文章(30)
Jean M. Sargent, The Use of the MTT Assay to Study Drug Resistance in Fresh Tumour Samples Recent results in cancer research. ,vol. 161, pp. 13- 25 ,(2003) , 10.1007/978-3-642-19022-3_2
Richard Fox, Martine Aubert, Flow cytometric detection of activated caspase-3. Methods of Molecular Biology. ,vol. 414, pp. 47- 56 ,(2008) , 10.1007/978-1-59745-339-4_5
D. Ge, K. Zhang, A. C. Need, O. Martin, J. Fellay, T. J. Urban, A. Telenti, D. B. Goldstein, WGAViewer: Software for genomic annotation of whole genome association studies Genome Research. ,vol. 18, pp. 640- 643 ,(2008) , 10.1101/GR.071571.107
R. Stephanie Huang, Shiwei Duan, Sunita J. Shukla, Emily O. Kistner, Tyson A. Clark, Tina X. Chen, Anthony C. Schweitzer, John E. Blume, M. Eileen Dolan, Identification of genetic variants contributing to cisplatin-induced cytotoxicity by use of a genomewide approach. American Journal of Human Genetics. ,vol. 81, pp. 427- 437 ,(2007) , 10.1086/519850
F Di Fiore, F Blanchard, F Charbonnier, F Le Pessot, A Lamy, M P Galais, L Bastit, A Killian, R Sesboüé, J J Tuech, A M Queuniet, B Paillot, J C Sabourin, F Michot, P Michel, T Frebourg, Clinical relevance of KRAS mutation detection in metastatic colorectal cancer treated by Cetuximab plus chemotherapy. British Journal of Cancer. ,vol. 96, pp. 1166- 1169 ,(2007) , 10.1038/SJ.BJC.6603685
Lieke Henriette Tanja, Henk-Jan Guchelaar, Hans Gelderblom, Pharmacogenetics in chemotherapy of colorectal cancer Best Practice & Research Clinical Gastroenterology. ,vol. 23, pp. 257- 273 ,(2009) , 10.1016/J.BPG.2009.02.011
Michael S Braun, Philip Quirke, Matthew T Seymour, Molecular markers of chemotherapeutic response and toxicity in colorectal cancer Expert Review of Anticancer Therapy. ,vol. 7, pp. 489- 501 ,(2007) , 10.1586/14737140.7.4.489
Liang Li, Brooke L. Fridley, Krishna Kalari, Gregory Jenkins, Anthony Batzler, Richard M. Weinshilboum, Liewei Wang, Gemcitabine and Arabinosylcytosin Pharmacogenomics: Genome-Wide Association and Drug Response Biomarkers PLoS ONE. ,vol. 4, pp. e7765- ,(2009) , 10.1371/JOURNAL.PONE.0007765
Jian Wang, Sanjay Shete, A test for genetic association that incorporates information about deviation from Hardy-Weinberg proportions in cases. American Journal of Human Genetics. ,vol. 83, pp. 53- 63 ,(2008) , 10.1016/J.AJHG.2008.06.010
R. A. Vescio, C. H. Redfern, T. J. Nelson, S. Ugoretz, P. H. Stern, R. M. Hoffman, In vivo-like drug responses of human tumors growing in three-dimensional gel-supported primary culture Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 5029- 5033 ,(1987) , 10.1073/PNAS.84.14.5029