Solid-State NMR Spectroscopy Provides Atomic-Level Insights Into the Dehydration of Cartilage

摘要: An atomic-level insight into the functioning of articular cartilage would be useful to develop prevention strategies and therapies for joint diseases such as osteoarthritis. However, composition structure their relationship its unique mechanical properties are quite complex pose tremendous challenges most biophysical techniques. In this study, we present an investigation dynamics polymeric molecules using time-resolved solid-state NMR spectroscopy during dehydration. Full-thickness explants were used in magic-angle spinning experiments monitor structural changes rigid mobile carbons. Our results reveal that dehydration reduced mobility collagen amino acid residues carbon sugar ring structures glycosaminoglycans but had no effect on trans-Xaa-Pro conformation. Equally interestingly, our demonstrate effects reversible, molecular an...